Studies of chemotaxis involving the mammalian neutrophils and breast cancer cells:
Tumor spheroid day 7 stained with calcein AM (green) and ethidium homodimer (red) imaged using light sheet microscopy
Fluorescently labeled neutrophils migrating directionally towards chemokine (placed righthand side) in 3D matrix of collagen. Video captured using LSM 880 confocal microscope using temperature controlled unit.
Studies of cancer cell and neutrophil migration influenced by tumor-immune cell cross talks